About us

What BISY BIOS is all about

is a spin-off company of bisy GmbH founded in 2020 by Claudia Rinnofner. We focus on the production of protein-based (biological) molecules with unique physical and biochemical properties. Our head-office is located in Hart bei Graz. In September 2021 bisy BIOS will move to its newly rented laboratory at ZWT (Zentrum für Wissens- und Technologietransfer in der Medizin, Neue Stiftingtalstraße 2 | 8010 Graz).



Claudia Rinnofner (CEO) earned her PhD at Graz University of Technology (TUG) in the research group of Prof. Anton Glieder at the Institute of Molecular Biotechnology. Her doctoral thesis was devoted to the expression of recombinant proteins in Pichia pastoris employing synthetic promoters. Following her PhD she worked at ACIB GmbH (Austrian Centre of Industrial Biotechnology) in an industrial multi-firm project. Claudia Rinnofner has more than 15 years experience as a scientist, working with Pichia pastoris and strain engineering including the recombinant expression of various heterologous proteins.



Felber, M., Pichler, H., Ruth, C., 2014. Strains and molecular tools for recombinant protein production in Pichia pastoris. Methods Mol. Biol. 1152, 87–111. https://doi.org/10.1007/978-1-4939-0563-8_5

Fürst, M., Kerschbaumer, B., Rinnofner, C., Migglautsch, A., Winkler, M., Fraaije, M., 2019. Exploring the Biocatalytic Potential of a Self-Sufficient Cytochrome P450 from <i>Thermothelomyces thermophila</i> Adv. Synth. Catal. https://doi.org/10.1002/adsc.201900076

Hartner, F.S., Ruth, C., Langenegger, D., Johnson, S.N., Hyka, P., Lin-Cereghino, G.P., Lin-Cereghino, J., Kovar, K., Cregg, J.M., Glieder, A., 2008. Promoter library designed for fine-tuned gene expression in Pichia pastoris. Nucleic Acids Res. 36, e76. https://doi.org/10.1093/nar/gkn369

Hashem, C., Stolterfoht, H., Rinnofner, C., Steinberger, S., Winkler, M., Pichler, H., 2020. Secretion of Pseudomonas aeruginosa Lipoxygenase by Pichia pastoris upon Glycerol Feed. Biotechnol. J. 15, 2000089. https://doi.org/10.1002/biot.202000089

Mellitzer, A., Ruth, C., Gustafsson, C., Welch, M., Birner-Grünberger, R., Weis, R., Purkarthofer, T., Glieder, A., 2014. Synergistic modular promoter and gene optimization to push cellulase secretion by Pichia pastoris beyond existing benchmarks. J. Biotechnol. 191. https://doi.org/10.1016/j.jbiotec.2014.08.035

Näätsaari, L., Mistlberger, B., Ruth, C., Hajek, T., Hartner, F.S., Glieder, A., 2012. Deletion of the pichia pastoris ku70 homologue facilitates platform strain generation for gene expression and synthetic biology. PLoS One 7. https://doi.org/10.1371/journal.pone.0039720

Rinnofner, C., Kerschbaumer, B., Weber, H., Glieder, A., Winkler, M., 2019. Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst. Biocatal. Agric. Biotechnol. 17, 525–528. https://doi.org/10.1016/J.BCAB.2018.12.022

Ruth, C., Buchetics, M., Vidimce, V., Kotz, D., Naschberger, S., Mattanovich, D., Pichler, H., Gasser, B., 2014. Pichia pastoris Aft1 – a novel transcription factor, enhancing recombinant protein secretion. Microb. Cell Fact. 13, 120. https://doi.org/10.1186/s12934-014-0120-5

Ruth, C., Glieder, A., 2010. Perspectives on synthetic promoters for biocatalysis and biotransformation. ChemBioChem 11. https://doi.org/10.1002/cbic.200900761

Ruth, C., Zuellig, T., Mellitzer, A., Weis, R., Looser, V., Kovar, K., Glieder, A., 2010. Variable production windows for porcine trypsinogen employing synthetic inducible promoter variants in Pichia pastoris. Syst. Synth. Biol. 4. https://doi.org/10.1007/s11693-010-9057-0

Stolterfoht, H., Rinnofner, C., Winkler, M., Pichler, H., 2019. Recombinant Lipoxygenases and Hydroperoxide Lyases for the Synthesis of Green Leaf Volatiles. J. Agric. Food Chem. https://doi.org/10.1021/acs.jafc.9b02690